Subcellular Optogenetics

We use optogenetic methods to spatially confine critical molecular to selected regions of a single cell. This approach allows cell migration, cytokinesis and neuron differentiation to be optically directed. The spectral selectivity of optogenetic tools allows intracellular molecules to be quantitatively imaged simultaneously using fluorecent proteins or fluorophores while orchestrating cell behavior. Using this approach we deconstruct complex cell behaviors to understand the mechanisms at their basis.