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The system has the following components:

1.     Leica DMI6000 automated scope with motorized XYZ axis; DIC optics; epifluorescence ready  – The scope is completely automated and controllable through attached computer. Inverted scope with 63x plan apochromat and 20x FluoR lenses. Multiple prism slots for visualizing multiple fluorophores through eye piece. The front panel switches allow unhindered control of various functions of the scope. The scope can also be independently operated without the  computer. Full color images from the scope can also be captured using a Nikon D40 SLR attached to the right photoport of the scope. The left photoport is attached to the laser spinning disk confocal unit. The XYZ axes are controlled using a isolated controller.

2.     Environment incubation chamber – Specially designed for live cell imaging studies, the ‘On-scope incubator’ maintains the cells at 37oC, optimal CO2 concentration and humidity for long imaging experiments.

3.     Yokogawa spinning disk confocal unit – especially useful  for live cell imaging studies on account of its ability to capture images at high speed without having undue photobleaching or phototoxicity effects. We possess multiple dichroics for imaging multiple fluorophores using this unit.

4.     Andor Revolution XD – A unit specifically designed for live cell confocal imaging with multiple laser lines. Integral device for combining multiple laser lines, their rapid  switching and multiport delivery which allows same laser lines to be used for confocal imaging, FRAP and photoactivation experiments and TIRF.

5.     Solid state lasers – Multiple laser lines for imaging a variety of fluorophores. We possess 50 mW solid state laser lines of 454nm, 488nm, 515nm and 594nm wavelengths. Solid state laser allow rapid initialization of the lasers; do not generate heat and noise while operational and have a long life (up to 10,000 hrs). We have lasers from - Coherent and Cobolt Inc.

6.     Andor iXon EM-CCD camera – Integral for spinning disk confocal imaging on account of its higher sensitivity. Resolution 512 x 512k; upto 35 frames per second at maximum resolution; 99% quantum efficiency; cooled to -100oC to eliminate dark noise/ current; dual chip sensor for rapid frame transfer features. Capable of single – molecule imaging experiments.

7.     Revolution FRAPPA – A specially designed unit for FRAP and photoactivation (PA) studies in living cells. Capable of FRAP and PA with 10 msec time frames. Uses a dual galvanometer scan head to provide a computer-steered laser beam delivery system. Uses a unique ‘point 'n shoot’ technique for FRAP and PA.

8.     Andor iQ – The software which runs all these units in perfect sync. User friendly and intuitive design. Uses a unique kinetic image disk for rapid acquisition and storing vast amounts of imaging data.

9.     Dell Optiplex workstation – Complementing the advanced imaging system, Intel Quad Core Duo processor; 4GB RAM; 1TB RAID 0 HDD; 2 TB secondary HDD; 30 inch digital monitor; wireless input devices; multiple external backup devices. Onboard DAC and laser controlled devices for generating rapid responses from attached devices.

10.  Vibration Isolation Table

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The system has the following components:

1.     Nikon TE2000 scope – Inverted scope with 63x plan apochromat; 20x and 10x lenses. Multiple prism slots for visualizing multiple fluorophores through eye piece. The left photoport is attached to a CCD camera.

2.     Exfo high intensity light source – High intensity white light source for imaging a variety of fluorophores using specific excitation and emission filters. We posses 100W unit.

3.     Hamamatsu ORCA-ER CCD camera – Integral for detecting low intensity fluorescence signals. Best camera in its class.

4.     Sutter high speed filter wheels – A specially designed unit for imaging studies in living cells. Filter wheels switch with 55msec allowing high speed imaging of multiple fluorophores.

5.     8 channel fluid delivery system – Allows precise control of the delivery of various solutions/ treatments of cells in the imaging chamber. We use a very low volume (25ml) imaging chamber for kinetic experiments. The fluid delivery system is completely programmable and can control the delivery of upto 8 solutions to the imaging chamber.

6.     Metamorph – The software which runs all these units in perfect sync. User friendly and intuitive design. Allows acquisition; analysis and post acquisition processing of imaging data.

7.     Dell Optiplex workstation – Complementing the advanced imaging system, Intel Core Duo processor; 4GB RAM; 1TB primary and secondary HDD; 24 inch digital monitor; wireless input devices; multiple external backup devices.

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The system has the following components:

1.     Zeiss Axioscope – Upright scope with 60x plan apochromat lens; 20x and 10x lenses. Multiple prism slots for visualizing multiple fluorophores through eye piece. The left photoport is attached to a CCD camera.

2.     Exfo high intensity light source – High intensity white light source for imaging a variety of fluorophores using specific excitation and emission filters. We posses 100W unit.

3.     Hamamatsu ORCA-ER CCD camera – Integral for detecting low intensity fluorescence signals. Best camera in its class.

4.     Sutter high speed filter wheels – A specially designed unit for imaging studies in living cells. Filter wheels switch with 55msec allowing high speed imaging of multiple fluorophores.

5.     8 channel fluid delivery system and perfusion system – Allows precise control of the delivery of various solutions/ treatments of cells in the imaging chamber. We use a very low volume (25ml) imaging chamber for kinetic experiments. The fluid delivery system is completely programmable and can control the delivery of upto 8 solutions to the imaging chamber.

6.     Metamorph – The software which runs all these units in perfect sync. User friendly and intuitive design. Allows acquisition; analysis and post acquisition processing of imaging data.

7.     Dell Optiplex workstation – Complementing the advanced imaging system, Intel Core Duo processor; 4GB RAM; 1TB primary and secondary HDD; 18 inch digital monitor; wireless input devices; multiple external backup devices.